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Jayesh

Jamsab computers is a Computer training institute from Ahmedabad
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r cells. Transfected ES cells underwent double-selection with the neomycin analogue G418 (Gibco), at a concentration of 200 mg/ml and with gancyclovir (2 mM). ES clones were screened by Southern blot analysis using DNA probes external to both the 59 and 39 ends of the Krt7 homology arms. Microinjection of ES cell clones and generation of chimeric mice were performed as described previously [14]. C
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